Cerebellum (20x objective lens)

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The left three-quarters of this image shows the three layers of gray matter in this part of the brain. The outer molecular layer (left third of the image) has a uniform structure containing a few, darkly stained cell nuclei. These nuclei belong to small interneurons, called stellate cells, and glia cells. The heavily stained granular layer contains numerous cells with purple-stained nuclei. In between is the Purkinje cell layer, which is one cell-layer thick, containing the very large perikarya of Purkinje cells. The pale-staining nuclei and prominent nucleoli of the Purkinje cells can be seen even at this magnification. The "holey" layer at the right edge of the image is the white matter of the cerebellum, consisting of nerve fibers and glia cells.

 

Stain = H&E.

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